Q Space!!

the serpent said 'take the fruit and you will be able to tell good and bad, like God.' Eve took the fruit and ate it. suddenly she felt ashamed of being naked. she then gave Adam the fruit too. He felt the same way. they both used leaves to cover their body so that they weren't embarrassed any more. God came, found the sin was committed. He then punished the serpent by crawling on the ground and breath in dust all the time. Woman was going to suffer pain from bearing children. Man had to work to keep the family going. the happy life in heaven was gone, forever.

i was confused that why God didn't want them to know what is good and what is bad. wouldn't that be a good education for them in the very beginning of being created? a wise friend explained this to me. Adam and Eve were like children, they sense no shame, they had no worry, they weren't judging, until, they grew up. the moment of finding your growing up can be scary. you then have to understand what one should and shouldn't do. be able to know good from bad. women mature and will be bearing children. men work, afford family. it is just like what is happening every minute all around the world. is that a good thing to grow up or not? well, it is a question that worthless. you cannot avoid it (might some one managed, lucky guys), and you will need to know how to face it. take the fruit, without hesitation, as it wasn't meant to be avoided. open your eyes and sense the pain. it is life, and meant to be lived.

if the experiments are short (< 60 s), an unexpected bleaching shouldn't not occur. fluo4 + Oregon green would actually be adequate . if the expeirments would last longer than 10 min, should ratio metric be required to use then.. that actually makes things rather clear --- at least now, for the pilot experiments, one type of indicator would be ok. later, if we need to establish a more accurate method, we can always employ a confocal microscope.

a new set of experiments are listed now ..it will be in combination of ramping uncaging calcium and exocytosis study.
currently there are a few technical difficulties to overcome.

1. the uncaging uv peaks at 365 nm and that is the same for fura-2 ca unbound form (in another word, it will excite fura2 and give more background noise)
2. fluo4 appears to be an option with the ex at 488 and em at 520. but its single ex/em makes the calibration complicated.
3. fura red would work for ratio metric but the kd is too low .. i.e. when ca excesses 300 nM , the signal will be saturated ..
4. what about confocol?

i have a fully occupied schedule now..
1. the camp/glucose manuscript has to be finished by the end of this month/beginning of next month
2. photo-liberation experiments for jochen lang
3. finish the nav ko experiments (it is urgent now)
4. trip to sweden by the end of nov
and 5. apply the wolfson college fellowship..
oh God.. it is hectic but i will manage!

a pretentious knows nothing yet thinks he knows everything. funny enough, i have met a person like this. a big talker and a dwarf in  action. i was pissed off by him because it was simply irritating to see him in front of me. yet, after a few moment of thinking, i thought, ' well, if you are pissed off by him, then you have lowered yourself in his range'. i stopped being furious and knowing what matters to me more. anyway, it is not pleasant at all to meet those people, but it will be good if you may simply laugh, and don't give a shite about him.

a few quotations:
1. q: if you are frustrated/upset at work, what will make you feel a bit better? and how are you going to sort the problem
    a:  i will grab a mate and tell him to f**k off
2. Q: if the other one is holding a different opinion, what will you do?
    A: force him to accept mine
3.  Q: people are born equal, isn't it?
    A: no. some one is much better than the others (i have no idea what 'better' refers to here)
4.  Q: Van Gogh was a great artist, isn't it?
    A: he is a piece of shit. his painting is just like a scramble of children. he is famous just because people made him so. (note: Van Gogh was pronounced as  van gorg. )
5.  Q : this restaurant is quite good, isn't it?
     A: oh.. pub is my thing, hold on, a dish cost 15 quid .. that is very pricey (oh my word, where are you from? that is just a normal normal dish)
6.   Q: like some wine?
      A oh. i know a famous vineyard in spain, and they will ... blah blah blah blah ....(God, give me a break, shall we start from some grapes first?)
7. Q: Asian people normally are not considered to be romantic, what you think?
     A: you are all wax face. do you know what wax mean (i have a phd, i guess i do.), you don't show expression when you speak. (F***ing hell, take a piss and try to look at yourself, your f***ing face shows nothing but stupidity. and i am afraid i have to fail you for this answer. we are trying to address romance, what is that to do with face/wax face? i meant normally, not absolutely. and taken the comments about black people you made earlier, i am sorry, you are a nazi, and that is not nice)
8. Q: what recent movie you like most?
      A: the libertine (johnny depp, oh finally there is something good coming). i like it because people spoke proper english in it. (?!! johnny depp?? is he american?oh yes, he is great actor too. he can do english accent..why don't we just go on the street and chat with some locals? aren't we in oxford anyway? oh sorry, i forgot, you are only a guy runs some machine, you are not an academic.. oh. by the way,  your accent  isn't nice at all... too artificial )


oh God, how could such a person crawl into my life? i certainly don't want to meet him again... but, he is keeping on showing up (from a couple of months ago that he invited himself to my party?!).. didn't he notice that i am disgusted upon his presence? alas..

well, again, as i said in the beginning of this blog.. the thing that one should do (if you can not get rid of him) is simply laughing at him (well, if you want, you may be sarcastic and make some witty jokes to embarrass him.. sorry, i know that is mean.. just can not help..well, next time, if he starts talking about Chopin/wine/literature, sorry, i will make you embarrassed.. and dare you not say anything about Van Gogh!! )

it is hard to know some one without traveling together.. and for me, this guy, one trip is much more than enough.. what a wanker..

1. entering the building
2. changing room (2nd floor)
3. find the right rig
4. pass 2 auto doors to get the box and saw dust
5. hood
6. transfer
7 hood off. go

feeling, different from knowing, is vague. most time you don't know what it is, although it is there. i gazed in the trees and clouds that are far away, i got a kind of feeling. i took a glimpse on the pretty girl passed by, i got a kind of feeling. i listen to the radio, i got a kind of feeling.. i don't know what it is, but i know how i feel. it some time is happy, some time is sad, some time is relaxing, and also can be stressful. okey doke.. i am not going to be such an sentimental boy for the moment--- i was discussing with an itallian girl about her handbag another day already.. where the hell are my balls..

now i am going back to the main topic.. it is quite interesting, just got hit by a kind of feeling. found myself calm and more sensible (although i have almost finished a bottle of wine already.. some time i worry that i will have drinking problem.. :-)  ) it is quite an interesting feeling, that i know what i want to do. (still, if you have read the first paragraph by any chance, you know the difference between knowing and feeling...) .. well, i don't think i shall disclose it here.. ( as it is a secret.. ;-P )but i can tell alll you lovely people who care about me and love me, it is a content and joyful feeling that i haven't felt for a long time. relaxed and drawing a big smile on my face..

a couple of trivia though.. some one secretly took care of my garden, the hedge and bush were taken a very descent care. the garden looks even better than before i moved in.
i am going to gym often now.. and i can row for 35 min (7 k) and run for 25 min (4 and half k)..
i am about to join the rowing team in wolfson college, hopefully it will be good.. okey, i am sure it will be good if i can get in.
i am quiting the taekwondo club in brooks as i found the training is a waste of time. a snail has just fallen of my hedge.
 i am going to the dance club for a try of salsa.. (maybe waltz as well?? not sure about it, although i am quite good with it.
right now a frog is hoping around me...  
i am going to plan a trip to paris very soon. lovely city and it is sooooo close.. blessed.
i am thinking about going to sweden for a vacation trip... lol.. meeting my old mates and my sis
my badminton is getting better and i can run quite a bit while i am playing.. although i got a sore butt today  (  i go to gym 3 times a week and i go jogging very often, i still get a sore butt from badminton? )
i sing (quietly) when i am on the rowing machine.. and when i am cooking
i chat with random strangers whom i met in the smoking corner..
i can sit in my garden for an hour to read the biography of Churchill (cool book, really like it and i like him now. .. it is a shame that his great grand nephew is such a wimp )
i have friends over now and then to join me for a chill with some wine and food..
i appreciate all my mates and people around.. and i don;t appreciate any rude people.. and they know it..
i am going to get up early tomorrow and get my work done early.
i keep my hope high for the next project as it will be AWESOME!!!

do they all sound good to you? and do you feel the way i feel? my dear friends?
finally, right now, i have a big smile on my face!!
q